行政院農業委員會台南區農業改良場   研究彙報第62號

 

利用簡單重複序列標記建立臺灣毛豆品種的鑑別技術

王聖善、黃詩婷、蔣雅文、王裕權、張嘉滿、楊藹華

摘  要

  王聖善、黃詩婷、蔣雅文、王裕權、張嘉滿、楊藹華。2013。利用簡單重複序列標記建立臺灣毛豆品種的鑑別技術。臺南區農業改良場研究彙報62:22-29。

  毛豆為近年來臺灣重點出口作物之一,為有效鑑別臺灣地區毛豆品種與大豆品種並保護其品種權,本研究以95個簡單重複性序列標誌,針對6個參試品種進行初步篩選,經PCR反應與電泳分析結果顯示,17組標誌於參試品種中具多型性。為了進一步建立精確之毛豆DNA指紋圖譜資訊,本研究將17組具多型性分子標誌進行引子螢光修飾,並針對14個毛豆品種、3個黑豆品種、2個綠肥大豆品種與1個黃豆品種進行連鎖聚合酶反應後,再以毛細管電泳進行片段長度分析,結果顯示,13組分子標誌具高PIC值與低殘跡條帶等優良特性。在品種親源鑑定方面,除了日本毛豆品種82茶豆與毛豆臺南選1號的分子標誌多型性模式完全相同外,其餘品種均可被鑑別,其最少只需GMES158、GMES6391、GMES6224與GMES4226等四組標誌即可鑑別19個參試品種。由以上結果顯示,此系統能夠精準且有效率的鑑別臺灣毛豆品種並保護其品種權。

關鍵字:毛豆、品種鑑定、簡單重複性序列
接受日期:2013年9月25日


Variety Identification among Major Vegetable Soybean Cultivars of Taiwan
Based on Simple Sequence Repeat Markers

Wang S. S. ,S. T. Huang, Y. W. Jiang, Y. C. Wang, C. M. Chang and A. H. Yang

Abstract

   Vegetable soybean is one of the most important crops for export in Taiwan. In order to establish a varietal verification system of vegetable soybean, 95 soybean SSR markers were designated and analyzed by using 6 cultivars. The result indicated that 17 SSR markers presented polymorphism among cultivars. To get an accurate fingerprinting databases, 20 soybean cultivars, including 14 vegetable soybeans, 3 black soybeans, 2 green-manure soybeans and one soybean cultivars were amplified with 17 polymorphic markers and assayed by using ABI-3730 capillary sequencing analysis. Eventually, 13 markers with low stutter band and high PIC value were acquired. According to the result of genetic distance analysis, only cultivar Tainan selected No.1 showed the same pattern with the Japanese cultivar 82 soybean. Moreover, only 4 markers, GMES158, GMES6391, GMES6224 and GMES4226 could identify 19 tested cultivars. All the result above suggested that this system could accurately verify most vegetable soybean cultivars in Taiwan.

Key words: Vegetable Soybean, Variety Identification, Simple Sequence Repeat
Accepted for publication: September 25, 2013


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