行政院農業委員會台南區農業改良場   研究彙報第52號

 

利用分子生物技術進行青花菜F1雜交品種純度鑑定

陳國憲、楊藹華

摘  要

  陳國憲、楊藹華。2008。利用分子生物技術進行青花菜F1雜交品種純度鑑定。台南區農業改良場研究彙報52:72-80。

  自UBC之100個ISSR選得24個引子,應用於12個生產F1雜交一代的青花菜親本DNA指紋分析,再選用其中最適的 UBC807、UBC808、UBC811、UBC823、UBC834、UBC836、UBC840、UBC841、UBC846、UBC855等10組引子進行PCR反應,得到的DNA指紋多型性,進行Hier- cluster樹狀圖分析,可將青花菜的12個親本依差異性大小區分為Br02、Br08、Br29、Br30、Br21;Br03、Br06、Br07、Br23、Br31、Br27 及Br05三大類群。檢測F1的種子純度時,將受測的不同親本組合:Br07×Br02、Br05×Br27、Br07×Br21、Br07×Br31、Br27×Br05、Br05×Br07、Br07×30等7個組合F1種子,分別以Br0702、Br0527、Br0721、Br0731、Br2705、Br0507、Br0730為代號。分析7個F1種子與其親本DNA指紋差異,結果Br0721、Br0730、Br0731、Br2705、Br0507等5個F1種子間純度可達100%;但在Br0702、Br0527 F1種子純度則較低,分別為94.3%及64.5%。

關鍵詞:青花菜、F1種子純度、ISSR


Application of ISSR Markers to Determined the Purity of F1 Seeds of Brassica oleracea var. botrytis L.

Chen, K. H.  and A. H. Yang

Abstract

  The DNA amplification using the ISSR's appeared high efficiencies of characterizing the male and female parent based on these specific bands; thus, it were successfully used to determine the purity of F1 seeds. In the experiment the twelve parents of Brassica oleracea var. botrytis L. could be distinguished by the specific bands based on the ISSR -PCR of 24 UBC primers; the genetic dendrogram of the parents constructed by Hier- cluster analysis, based on the specific bands these parents genotypes were grouped into 3 major distinct clusters from UBC807, UBC808, UBC811, UBC823, UBC834, UBC836, UBC840, UBC841, UBC846, UBC855 primers. In the purity analysis of 7 F1 hybrids of Br0702, Br0527, Br0721, Br0731, Br2705, Br0507, and Br0730 .The results found that the seed purities of Br0721, Br2730, Br0731, Br2705, Br0507 hybrids had appeared 100% purity, but Br0702, Br0527 had lower purity were 94.3% & 64.5%, respectably.

Key words : Brassica oleracea var. botrytis L., F1 hybrid purity,  ISSR (inter simple sequence repeats)


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