行政院農業委員會台南區農業改良場   研究彙報第50號

 

黑豆及毛豆未熟子葉培養體胚增殖及分化之研究

王瑞章、葉茂生

摘  要

王瑞章、葉茂生•2007•黑豆及毛豆未熟子葉培養體胚增殖及分化之研究。台南區農業改良場研究彙報50:1-8。
利用栽培種大豆(Glycine max(L.)Merr.)黑豆台南三號(TN3)及毛豆高雄選一號(KS1)之未成熟種子(長度4-5mm)之子葉,接種於以MSB為基礎培養基,各添加40mg/L 2,4-D及8g/L agar(a)或2g/L gelrite(g),以及再各添加30g/L(M2a,M2g),60g/L sucrose(M3a,M3g)等4種培養基誘導的胚性組織與體胚,將胚性組織團塊繼代於FN Lite培養基,進行胚性懸浮培養,探討對黑豆及毛豆誘導體胚增殖及分化之影響,期能建立栽培種黑豆及毛豆之體胚培養技術,結果如下︰懸浮培養對黑豆及毛豆體胚增殖之效果,球形胚增殖倍率黑豆台南三號為2.8倍,優於毛豆高雄選一號之1.8倍。每個胚性組織平均形成球形胚數黑豆台南三號為3.2個,毛豆KS1則為2.6個。
關鍵詞︰黑豆、毛豆、未熟子葉、體胚分化
接受日期:2007年 12 月4日


In Vitro Somatic Differentiation of Immature Cotyledon of Black Soybean and Vegetable Soybean

Wang,R.C. and M.S. Yeh

Summary

The purposes of the experiments that are to establish a tissue culture system for somatic embryogenesis from immature cotyledon of soybean, and the results will be applied on inducing transgenic plant from explant and embryonic rescue of plant breeding. The explants, immature cotyledons of cv. TN3 of black soybean and cv.KS1 of vegetable soybean, were cultured on modified MSB basal medium containing 2,4-D and other nutrients to induce somatic embryo.The results are summarized as follows : The formations ratioes of somatic embryos with suspension culture of cv. KS1 and cv. TN3 were 2.8 and 1.8, respectively, and the formations ratios of globular embryos were 2.6 and 3.2, respectively. Key words:black soybean , vegetable soybean , immature cotyledon , somatic differentiation Accepted for publication:4 December,2007


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