行政院農業委員會台南區農業改良場   研究彙報第49號

 

應用分子生物技術 PCR-RFLP分析花椰菜雜交品種之親本自交不親和類型

陳國憲、楊藹華、王仕賢

摘  要

陳國憲、楊藹華、王世賢。 2007。應用分子生物技術PCR-RFLP分析花椰菜雜交品種之親本自交不親和類型。 台南區農業改良場研究彙報 49:56-65。

   本研究運用分子生物技術 PCR-RFLP方法分析花椰菜38個生產雜交品種的親本自交不親和基因型類型的異同。選擇對花椰菜自交不親和具專一性高之引子組合BS1及BS2,並以花椰菜雜交親本DNA模板,進行聚合?連鎖反應,以獲得一分子量約1154 bp之自交不親和DNA片段。將此一DNA分別以六種不同限制?反應後,再以電泳進行分析後,根據其在不同限制?反應所產生圖譜差異,來區分親本間的自交不親和基因類型異同。在這ㄧ個實驗中受檢測的38個親本,可區分出至少有13個不同自交不親和基因型存在。

關鍵字: 花椰菜、自交不親和、 PCR-RFLP
接受日期: 2007年7月23日


Establish the S-alleles of Parent line of Commercial F1 Hybrid in
Brassica oleracea
var .botrytis linn . by PCR-RFLP Molecular Technique

Chen, K. H., A. H. Yang and S. S. Wang

Summary

  In this study, PCR-RFLP, a molecular method for the identification of S -alleles involved in self-incompatibility was used to analyse the S -alleles of 38 commercial parent line of Brassica oleracea var. botrytis Linn . The method consists of amplifitying by polymerase chain reaction sequences belonging to the S multigenes sequence family using a single pair of conserved primers ( BS1 、 BS2 ) . A single PCR product of predicted size of 1154 bp was obtained using genomic DNA for each of the 38 commercial parent line. PCR products are then analyed further by digestion with six restriction enzymes followed by gel electrophoresis. With any one restriction enzyme, several alleles showed the same restriction pattern. Alleles could therefore be grouped together. In Accordance with this method ,it showed 13 different S -allele type in the 38 commercial parent lines.

Keywords: Brassica oleracea var. botrytis Linn . , self-incompatibility, PCR-RFLP .

Accepted for publication: 23 July, 2007


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