Genetic Diversity of Garlic (Allium sativum) Varieties/Lines in Taiwan
Chao, H. F. and A. H. Yang
This study was attempted to explore the genetic diversities among 26 varieties/line of garlic (Allium sativum L.) obtained in Taiwan. Based on the characteristics of the external morphology, the coefficient of similarity ranged from 0 to 0.85 among the 26 varieties/lines. They also can be divided into two groups depending upon the systemic phylogeny analysis. The first group or soft-neck type, can be divided into two subgroup. The second group is hard-neck type. Big difference in similarity coefficient was observed between Ho-mei-fan (code 32) and any other varieties/lines in this group.
Different fragments of nucleic acid for the two groups can be obtained by the primer OPI03 with RAPD method. When the primer OPI03-3 was used for further analysis, the difference between the two groups of soft and hard-types could easily be obtained.
One more 800bp strip band of nucleic acid fragments was observed in all soft-neck varieties/lines only. The two varieties, Si-lo-pai-yeh (code 17) and Ho-mei-pai-yeh (code 19) were traditionally considered as soft-neck types. However, based on the results of the systemic phylogeny analysis and RAPD tests, no 800bp band of nucleic acid fragments was obtained, most of nucleic acid was between 900 and 1,800 bp. Therefore, they were considered as hard-neck types in this study, and in reality, they were the varieties in–between the two types.
Key words: garlic, genetic diversity, RAPD, Allium sativum
Accepted for publication: October 25, 2004