行政院農業委員會台南區農業改良場  研究彙報第43號

蔗糖對花生花藥癒合組織誘導及分化的影響

曾銀位、王瑞章、葉茂生

摘  要

曾銀位、王瑞章、葉茂生。2004。蔗糖對花生花藥癒合組織誘導及分化的影響。台南區農業改良場研究彙報43:18~27。

以栽培種花生(Arachis hypogaea L.) 台南11號 (TN11) 及台農5號 (TN5)二品種之單核期花藥為培植體,利用MS配方為基礎培養基,添加2mg/l 2,4-D及0.5 mg/l kinetin,再各分別添加30、60、90g/l蔗糖,及8g/l agar 等三種培養基(代號分別為D30、D60、D90):TN11及TN5二品種在3種培養基癒合組織之誘導率均佳。TN11癒合組織之形成率隨蔗糖濃度增加而降低(92.2%,92.1%及83.2%),而TN5卻隨蔗糖濃度增加而增加(76.7%,89.3%及94.6%),但二品種均以60g/l蔗糖時有最大的生長指數。TN11及TN5於含60 g/l蔗糖之D60培養基培養30天後,可產生體胚,其誘導率僅為2.0%,切片顯示體胚已發育至魚雷形期。而2品種在3種培養基均可形成白色突起,切片顯示白色突起為分化之根,內部已有維管束組織的分化

關鍵詞:花生、花藥培養、體細胞胚、體胚形成、分化

接受日期﹕2004年1月16日


Effects of Sucrose on Callus Induction and Differentiation from Anther Culture in Arachis hypogaea L.

Tseng Y. W., R. C.Wang, and M. S. Yeh

Summary

Anthers at uninucleate stage of two cultivars, Tainan No.11 (TN11) and Tainong No. 5 (TN5) cultured on MS basal medium containing 2 mg/l 2,4-D and 0.5 mg/l kinetin with addition of 30, 60, or 90 g/l sucrose , respectively.

All the three media gave successful induction of callia , and showed that , when the level of sucrose increased, the callus induction rate decreased (92.2% , 92.1% and 83.2%) in TN11 , while the rate increased (76.7% , 89.3% and 94.6%) in TN5. Both TN11 and TN5 had the heightest growth index of callus in D60 medium.After thirty days, both TN11and TN5 cultured on D60 medium obtained the 2.0% of somatic embryo induction rate. When anthers of TN11 on D60 and D90 media, or TN5 on D30, D60 or D90 media, there produced the white protrusions. Observation by parraffin section showed that the somatic embryo stage had developed to torpedo stage and the white protrusions were the differentiated roots with vascular system inside.

Key wordsArachis hypogaea (peanut) , Anther culture , Embryogenesis , Differentation.

Accepted for publication:16 January, 2004



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