行政院農業委員會台南區農業改良場  研究彙報第41號
 

黑豆及毛豆花藥培養之研究

王瑞章、謝桑煙、葉茂生

摘  要

王瑞章、謝桑煙、葉茂生。2003。黑豆及毛豆花藥培養之研究。台南區農業改良場研究彙報41:35~43。

利用栽培種大豆 (Glycine max (L.) Merr.) 黑豆台南3號 (TN3)、台南5號 (TN5),及毛豆高雄選1號 (KS1)、高雄5號 (KS5) 四個品種為材料。觀察花粉發育與利用MS為基楚培養基,各添加1 mg/l 2,4-D+0.1 mg/l TDZ (代號D101),2 mg/l 2,4-D+0.1 mg/l TDZ (D201),3 mg/l 2,4-D+0.1 mg/l TDZ (D301),1 mg/l 2,4-D+0.5 mg/l TDZ (D105),2 mg/l 2,4-D+0.5 mg/l TDZ (D205),3 mg/l 2,4-D+0.5 mg/l TDZ (D305),1 mg/l 2,4-D+1.0 mg/l TDZ (D110),2 mg/l 2,4-D+1.0 mg/l TDZ (D210),3 mg/l 2,4-D+1.0 mg/l TDZ (D310)等9種培養基,探討對黑豆及毛豆花藥癒合組織之誘導及體胚分化之效果,期建立黑豆及毛豆花藥培養技術,其結果如下:1.花藥發育階段中單核期之花蕾長度黑豆台南3號、台南5號為1.98-2.01mm,毛豆高雄選1號為1.96-1.98mm,高雄5號為1.98-2.0mm,因此黑豆及毛豆花藥培養最適宜之花蕾長度為1.96-2.0mm。2. 黑豆及毛豆花藥癒合組織以D201培養基所誘導之形成率最高。D201培養基對TN3、TN5、KS1及KS5的平均誘導率為31.6%,皆高於其它八種培養基,其次依序為D210培養基(30.2%),D310培養基 (27.0%),D110培養基 (26.8%),D301培養基 (25.3%),D105培養基(20.3%),D101培養基 (18.8%),而D305培養基最低 (17.5%)。 3. TN3於D101培養基中誘導體胚形成率為1.7%,KS1於D201培養基誘導體胚形成率為1.6%。

關鍵詞:黑豆、毛豆、花藥培養、體胚形成

接受日期:2002年12月27日


Studies on the Anther Culture of Black Soybean and Vegetable Soybean

Wang, R. C., S. Y. Hsieh and M. S. Yeh

Summary

    In order to develop the method of anther culture to produce haploid plants of black soybean and vegetable soybean , We investigated pollen development, callus induction and somatic embryogenesis. Anthers from black soybean cultivars TN3、TN5,and vegetable soybean cultivars KS1 、 KS5 were cultured on MS basal medium with nine different treatments, i.e., by the addition of (1)1mg/l 2,4-D+0.1mg/l TDZ(D101 Medium),(2)2mg/l 2,4-D+0.1mg/l TDZ (D201 Medium),(3)3mg/l 2,4-D+0.1mg/l TDZ (D301 Medium),(4)1mg/l 2,4-D+0.5mg/l TDZ (D105 Medium),(5)2mg/l 2,4-D+0.5mg/l TDZ (D205 Medium),(6)3mg/l 2,4-D+0.5mg/l TDZ (D305 Medium),(7)1mg/l 2,4-D+1.0mg/l TDZ (D110 Medium),(8)2mg/l 2,4-D+1.0mg/l TDZ (D210 Medium) or (9)3mg/l 2,4-D+1.0mg/l TDZ (D310 Medium) respectively. Results were summarized as follow:

    The lengths of buds of TN3、TN5、KS1 and KS5 at uninclate stage were about 1.98-2.01mm、1.98-2.01mm、1.96-1.98mm and 1.98-2.0mm, respectively. The results indicated that buds about 1.96-2.0mm in length was appropriate for culture. 

The rate of callus induction on the D201 medium was the highest. When average over the four test cultivars, rate of callus induction on the D201 medium was the highest (31.6%),followed by that on the D210 medium (30.2%), D310 medium (27.0%), D110 medium (26.8%), D301 medium (25.3%), D105 medium (20.3%), D101 medium (18.8%),and the D305 medium (17.5%) , respectively .

  For somatic embryos, the rates of induction on D101 media of TN3 and D201 media of KS1 were 1.7% and 1.6%, respectively .

Key words: Black soybean, Vegetable soybean, Anther culture, Somatic embryogenesis

Accepted for publication: 27 December, 2002



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